ironjustice
2011-10-03 02:00:59 UTC
Effect of polyunsaturated fatty acids on drug-sensitive and resistant
tumor cells in vitro
Undurti N Das1,2,3* and N Madhavi2
* Corresponding author: Undurti N Das ***@hotmail.com
1 Jawaharlal Nehru Technological University, Kakinada-533 003, India
2 UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH
44120, USA
3 Bio-Science Research Centre, Gayatri Vidya Parishad College of
Engineering,
Visakhapatnam-530 048, India
Lipids in Health and Disease 2011, 10:159 doi:10.1186/1476-511X-10-159
Published: 14 September 2011
This is an Open Access article distributed under the terms of the
Creative Commons Attribution License (http://creativecommons.org/
licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly
cited.
Abstract
Previous studies showed that γ-linolenic acid (GLA, 18: 3 ω-6),
arachidonic acid (AA, 20:4 ω -6), eicosapentaenoic acid (EPA, 20: 5 ω
-3) and docosahexaenoic acid (DHA, 22:6 ω -3) have selective
tumoricidal action.
In the present study, it was observed that dihomo-gamma-linolenic acid
(DGLA) and AA, EPA and DHA have cytotoxic action on both vincristine-
sensitive (KB-3-1) and resistant (KB-ChR-8-5) cancer cells in vitro
that appeared to be a free-radical dependent process but not due to
the formation of prostaglandins, leukotrienes and thromboxanes.
Uptake of vincristine and fatty acids was higher while their efflux
was lower in KB-3-1 cells compared with KB-ChR-8-5 cells, suggesting
that drug resistant cells have an effective efflux pump. GLA, DGLA,
AA, EPA and DHA enhanced the uptake and decreased efflux in both drug-
sensitive and drug-resistant cells and augmented the susceptibility of
tumor cells especially, of drug-resistant cells to the cytotoxic
action of vincristine.
These results suggest that certain polyunsaturated fatty acids have
tumoricidal action and are capable of enhancing the cytotoxic action
of anti-cancer drugs specifically, on drug-resistant cells by
enhancing drug uptake and reducing its efflux.
Thus, polyunsaturated fatty acids either by themselves or in
combination with chemotherapeutic drugs have the potential as anti-
cancer molecules.
The electronic version of this article is the complete one and can be
found online at: http://www.lipidworld.com/content/10/1/159
Lipids in Health and Disease 2011, 10:159 doi:10.1186/1476-511X-10-159
Received: 31 August 2011
Accepted: 14 September 2011
Published: 14 September 2011
© 2011 Das and Madhavi; licensee BioMed Central Ltd.
----------
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Tom
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http://tinyurl.com/634q5a
Man Is A Herbivore!
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tumor cells in vitro
Undurti N Das1,2,3* and N Madhavi2
* Corresponding author: Undurti N Das ***@hotmail.com
1 Jawaharlal Nehru Technological University, Kakinada-533 003, India
2 UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH
44120, USA
3 Bio-Science Research Centre, Gayatri Vidya Parishad College of
Engineering,
Visakhapatnam-530 048, India
Lipids in Health and Disease 2011, 10:159 doi:10.1186/1476-511X-10-159
Published: 14 September 2011
This is an Open Access article distributed under the terms of the
Creative Commons Attribution License (http://creativecommons.org/
licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly
cited.
Abstract
Previous studies showed that γ-linolenic acid (GLA, 18: 3 ω-6),
arachidonic acid (AA, 20:4 ω -6), eicosapentaenoic acid (EPA, 20: 5 ω
-3) and docosahexaenoic acid (DHA, 22:6 ω -3) have selective
tumoricidal action.
In the present study, it was observed that dihomo-gamma-linolenic acid
(DGLA) and AA, EPA and DHA have cytotoxic action on both vincristine-
sensitive (KB-3-1) and resistant (KB-ChR-8-5) cancer cells in vitro
that appeared to be a free-radical dependent process but not due to
the formation of prostaglandins, leukotrienes and thromboxanes.
Uptake of vincristine and fatty acids was higher while their efflux
was lower in KB-3-1 cells compared with KB-ChR-8-5 cells, suggesting
that drug resistant cells have an effective efflux pump. GLA, DGLA,
AA, EPA and DHA enhanced the uptake and decreased efflux in both drug-
sensitive and drug-resistant cells and augmented the susceptibility of
tumor cells especially, of drug-resistant cells to the cytotoxic
action of vincristine.
These results suggest that certain polyunsaturated fatty acids have
tumoricidal action and are capable of enhancing the cytotoxic action
of anti-cancer drugs specifically, on drug-resistant cells by
enhancing drug uptake and reducing its efflux.
Thus, polyunsaturated fatty acids either by themselves or in
combination with chemotherapeutic drugs have the potential as anti-
cancer molecules.
The electronic version of this article is the complete one and can be
found online at: http://www.lipidworld.com/content/10/1/159
Lipids in Health and Disease 2011, 10:159 doi:10.1186/1476-511X-10-159
Received: 31 August 2011
Accepted: 14 September 2011
Published: 14 September 2011
© 2011 Das and Madhavi; licensee BioMed Central Ltd.
----------
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://tinyurl.com/634q5a
Man Is A Herbivore!
http://tinyurl.com/4rq595
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk